Calcium gluconate is used to provide an energy boost to weakened and/or anorexic frogs. Supplied solids can be stored at room temperature indefinitely. Before use, dissolve solids by filling supplied dropper to top with pure water. Mix until dissolved. Add several drops of the solution to the frog’s back. This can be repeated several times a day. Store solution in the refrigerator during use. Freeze solution for long term storage. Discard if growth is evident. Note: substrate should be changed frequently during treatment as the glucose promotes bacterial and fungal growth.